Characterization of Deoxyribonucleases

نویسندگان

  • C. JUNGWIRTH
  • J. LAUNER
  • G. DOMBROWSKI
  • I. HORAK
چکیده

Increases in deoxyribonuclease activity assayed at alkaline pH can be observed in poxvirus-infected cells when native or denatured deoxyribonucleic acid (DNA) is used as substrate. The deoxyribonuclease assayable with native DNA as substrate, induced in HeLa cells by cowpoxvirus or vaccinia virus WR, can be separated from the corresponding enzyme present in normal cells by chromatography on diethylaminoethyl cellulose. In addition, the two enzymes induced in the virusinfected cells differ from each other in their chromatographic properties. The two induced enzymes have been further characterized with respect to properties of enzymatic reaction.

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تاریخ انتشار 2003